Extracellular Vesicle Core
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Welcome
Welcome to Children's Hospital of Los Angeles FBS Online Portal. This site is designed to automate the use of our Core Facilities and to provide the best possible customer service. Quick Info
For more info, please contact the Priority Software Support Team. | Our Core Facilities To learn more about a particular facility or to request access, please click on a facility name below. |
Main Contact Info
Paolo Neviani, PhD
4546 Sunset Blvd.
SRT room 215
Los Angeles, CA 90027
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The following Products and Services are available within our facility:
Sample Submission
Sample submission |
Services
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For more details click here
For inquiries contact: pneviani@chla.usc.edu More... |
Instruments
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NanoSight NS300 (For trained users only)The Malvern Panalytical NanoSight NS300 uses the technology of Nanoparticle Tracking Analysis (NTA).
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Ultracentrifuge (For trained users only)The use of the ultracentrifuge is complimentary. The users should be added to their PI's IBC protocol. Please provide current IBC approval memo. Training is required for unassisted use.
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Akta (For trained users only)Users that request access to the instrument will be able to independently perform a variety of chromatograpic applications, including but not limited to:
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Bio-Rad CFX96 (For trained users only)This sytem can be used for 96 well based quantitative real-time PCR experiments. Training is required for unassisted use.
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Tangential Flow Filtration (For Trained Users Only)The use of the tangential flow filtration system is complimentary. Training is required for unassisted use.
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Products
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Vesicle-depleted FBSBecause serum sources contain small extracellular vesicles of the origin species the use of a vesicle-depleted serum is recommended. More... |
CoreConnect Job Aids
CoreConnect Resources & Job AidsCHLA's Core Facilities at your finger tipsA web-based Core Management System that centralizes service and equipment scheduling, billing and usage tracking of CHLA’s nine centralized Core Facilities. Click here for resources & job aids: CoreConnect SharePoint More... |
Resources For Researchers
CHLA Core Pilot Grant program |
Core Use Policy
Review the Core Use PolicyThe general Core Use Policy includes essential guidance on regulatory compliance, scheduling a service, equipment use, paying for Core services, and Core acknowledgement in publications. More... |
This work was performed with the support of the ExtraCellular Vesicle Core at The Saban Research Institute of Children's Hospital Los Angeles.
Publications made possible by Core support:
- Xin Tang, Cheng Chang, Jiacong Guo, Vadim Lincoln, Mei Chen, David T. Woodley, and Wei Li. Department of Dermatology and the Norris Comprehensive Cancer Centre, University of Southern California Keck Medical Centre. Tumour-Secreted Hsp90a on the External Surface of Exosomes Mediates Tumour-Stromal Cell Communication via Autocrine and Paracrine Mechanisms. Sci Rep. 2019 Oct 22;9(1):15108
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6805946/
- Paolo Neviani, Petra M Wise, Mariam Murtadha, Cathy W Liu, Chun-hua Wu, Ambrose Y Jong, Robert CSeeger and Muller Fabbri Natural Killer-derived exosomal miR-186 inhibits neuroblastoma growth and immune escape mechanisms. Cancer Res December 12 2018 DOI: 10.1158/0008-5472.CAN-18-0779
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428417/
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